Journal: Cancer Science
Article Title: Copy number gain of ACTN4 is associated with poor prognosis in patients with upper urinary tract urothelial carcinoma
doi: 10.1111/cas.15841
Figure Lengend Snippet: Representative findings of fluorescence in situ hybridization for α‐actinin4 (ACTN4). Red and green signals indicate the ACTN4 and CEN19p signals, respectively. The figure shows the 4,6‐diamidino‐2‐phenylindone‐counterstained interphase nuclei for each specimen. (A) A case showing amplification as well as a higher copy number of ACTN4 . (B) A case showing increased numbers of both red and green signals, indicating a higher copy number but no amplification of ACTN4 (original magnification ×1000).
Article Snippet: ACTN4 SpectrumOrange/Chromosome 19 DNA probe ( CEN19p ) SpectrumGreen DNA probes (Abnova Corporation) were used for FISH analysis according to the same method as that of the PathVysion DNA probe kit (Abbott Molecular), as described previously.
Techniques: Fluorescence, In Situ Hybridization, Amplification